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KMID : 0364820080440010080
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Korean Journal of Microbiology
2008 Volume.44 No. 1 p.80 ~ p.84
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Construction of spDbp5 Null Mutants Defective in mRNA Export
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Bae Jin-Ah
Cho Hyun-Jin
Yoon Jin-Ho
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Abstract
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We constructed the null mutants of fission yeast Schizosaccharomyces pombe spDbp5 gene that is homologous to DEAD-box RNA helicase DBP5 in budding yeast Saccharomyces cerevisiae, which plays important roles in mRNA export out of nucleus. A null mutant in an h+/ h+ diploid strain was constructed by replacing the spDbp5- coding region with an ura4+ gene using one-step gene disruption method. Tetrad analysis showed that the spDbp5 is essential for vegetative growth. The haploid spDbp5 null mutants harboring pREP81X-spDbp5 plasmid showed extensive poly(A)+ RNA accumulation in the nucleus and decrease in the cytoplasm after repression of spDbp5 expression. These results suggest that spDbp5 is also involved in mRNA export from the nucleus.
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KEYWORD
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deletion mutant, mRNA export, Schizosaccharomyces pombe, spDbp5
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